《植物生理学报》 2011, 47(3): 263-268

拟南芥白化突变体EMS30的基因定位与分析

孙跃, 杨仲南, 崔永兰*

上海师范大学生命与环境科学学院, 上海200234

通信作者：崔永兰；E-mail: ylcui@shnu.edu.cn；Tel: 021-64324650

摘　要：

EMS30是拟南芥经甲基磺酸乙酯(EMS)诱变得到的白化突变体。该突变体的叶绿体结构存在严重缺陷, 同时伴随叶 绿素缺失。遗传分析显示EMS30突变体的突变表型受隐性单基因控制。采用图位克隆的方法对EMS30突变基因进行定位 的结果显示, 该基因位于拟南芥第一条染色体的分子标记F21M12和F14N23之间的96 kb区间内, 该区间包含25个基因。通 过生物信息学分析发现, 该区间内有3个基因定位在叶绿体或与叶绿体发育相关。这些结果有助于该基因的克隆, 为阐释 叶绿体发育提供线索。

关键词：拟南芥; 白化; 图位克隆

收稿：2010-11-08   修定：2011-02-24

资助：国家 “973” 项目(2009CB118504)。

Genetic Mapping and Analysis of Albino Gene EMS30 in Arabidopsis thaliana

SUN Yue, YANG Zhong-Nan, CUI Yong-Lan*

College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China

Corresponding author: CUI Yong-Lan; E-mail: ylcui@shnu.edu.cn; Tel: 021-64324650

Abstract:

An Arabidopsis thaliana albino mutant EMS30 was obtained by ethyl methane sulphonate (EMS) mutagenesis strategy. EMS30 showed severe defects in chloroplast ultrastructure in concomitance with chlorophyll deficiency. Genetic analysis indicated that albino phenotype was controlled by a single recessive locus. Using map-based cloning technique, EMS30 has been mapped to a region of 96 kb between molecular markers F21M12 and F14N23 on chromosome 1. In this region there are 25 genes, of which 3 genes are related with chloroplast function by bioinformatic analysis.

Key words: Arabidopsis thaliana; albino; map-based cloning